The 3500 series genetic analyzers set a new standard in capillary. Applied biosystems 3500 genetic analyzer biosurplus. Specification sheet 3500 and 3500xl series systems. Applied biosystems 3500 genetic analyzer from thermo fisher. System software primary analysis with 3500 series data collection software the applied biosystems 3500 and 3500xl genetic analyzers include data collection software with a simple user interface and clean design for easy display of consumable and array usage information, quickstart functionality, system maintenance reminders, and several. Solutions to issues related to software, data, hardware, and consumables miscommunication between the software. For comparison of energy usage when performing a run, the 3500xl instrument was set up to run on nominal voltage, 117. The 3500 series data collection software integrates seamlessly with. The 3500 series genetic analyzers1 are specifically designed to support the demanding performance needs of validated and regulated environments while retaining the unsurpassed application versatility that life science researchers expect from applied biosystems. For the applied biosystems 3500 series genetic analyzer, you can defragment the c and d drive only if the genemapper software has not been installed on the system. Rfid system of 3500 series which performs automatic detection of reagents and instrument accessories used, forces the instrument operators to replace these reagents and accessories unnecessarily frequently, nonefficiently and makes the whole operation of 3500. Reagents, supplies and equipment needed for genetic analyzer 3500 4. Finally, a series of selfpaced elearning modules are now available online, free of charge.
The 3500xl genetic analyzer has increased the number of capillaries used, allowing one to run more samples per injection while reducing the time of a run. With a throughput of up to 424 samples per day and the ability to upgrade from 8 to 24 capillaries, this platform provides the utmost in efficiency while still maintaining. The fourth digit is an internal number used by the software. Innovative software, hardware, and consumable development for. Perform comparative sequencing, linkage analysis, str analysis. Applied biosystems 35003500xl genetic analyzer user guide with 3500 series data collection software 3. The 8capillary 3500 and 24capillary 3500xl genetic analyzers. Maintenance tasks on the applied biosystems 3500 series genetic analyzer are broken down into daily, weekly, monthly, and asneeded tasks. If the system has been restarted and the computer slowdown is limited to the instrument data collection software. Do i need to replace the buffer every 14 days if the applied biosystems 35003500xl genetic analyzer is not in use. Troubleshooting will be conducted during the software training. This instrument is the stateoftheart platform for capillary electrophoresis of dna sequences for both nuclear and mitochondrial dna. Regulatory affairs specialist 5781 van allen way trade. An overview of the powerplex fusion system protocol.
Upgrade of applied biosystems genetic analyzers, 3500 series. Thermo fisher scientific provides wide range of products under applied biosystem brand which includes 3500 genetic analyzer. The 3500 8capillary and the 3500xl 24capillary genetic analysis systems have improved the industry standard for ce by providing greater throughput, flexibility and easeof use. Click spatial under the calibrate heading on the left of the navigation.
The complete system combines the instrument with applied biosystems reagents, consumables, and software, as well as. B genemapper id software applied biosystems 3 or 3xl genetic analyzer section 5. The 3500 series genetic analyzers set a new standard in capillary electrophoresis, with increased throughput, faster run times, and an innovative consumables system approach. With breakthrough hardware design, a whole new approach to consumables, and powerfulenabling software, the 3500 series delivers new levels of performance and convenienceto the work that you do every day. S eqstudio genetic analyzer for human identification.
The 3500 series instruments are the first genetic analyzers designed with a specific feature set and workflow for the human identification application. The reactions contained 4 ng of dna control 007 with the verifiler express pcr amplifi cation kit, and 1 ng of dna control 007 with the other kits. In capillary electrophoresisbased systems, dna fragments bound to probes migrate through a polymer and the fluorescence emissions are measured. Thermo fisher scientific life 3500xl genetic analyzer. The genetic analysis platform by which all sequencing technology is compared for accuracy and quality of data generation continues to be the capillary electrophoresis ce platform.
The pop7 separation matrix is optimized for short to longread sequencing and fragment analysis. The applied biosystems 3500 series genetic analyzer is designed to maintain the unsurpassed application versatility that life science researchers expect from applied biosystems instruments. The 3500 genetic analyzer is an 8capillary sequencing instrument specifically designed to support the demanding performance needs of validated and process controlled environments, while retaining the unsurpassed application versatility that life science researchers expect. After data collection, sample files are generated for each capillary and primary analys is is performed on these samples. Announcing new training options for the 3500 genetic analyzer. On going validation of the 3500xl genetic analyzer using the. The 3500 genetic analyzer is an 8capillary sequencing instrument specifically designed to support the demanding performance needs of validated and process controlled environments, while retaining the. The 3500 series genetic analyzers are optimized for validating ngs discoveries and performing translational research. Applied biosystems pop7 polymer for 35003500xl genetic.
System components the applied biosystems 3500 and 3500xl genetic. With breakthrough hardware design, a whole new approach to consumables, and powerfulenabling software, the 3500. The 3500 series data collection software supports sequencing and fragment. See users manual or package insert for limited label license, and trademark information. Technology on campus 3500 genetic analyzer youtube. For the 3500 genetic analyzer, our elearning training modules cover a wide range of topics including fragment analysis. Applied biosystems 3500 series data collection software 2abi 3500 3500xl genetic analyzer control software version 2new in box. Dna sequencers genetic analyzers genetic analyzers are automated systems capable of sequencing dna or analyzing fragments for a variety of applications.
For information on the 3500 series data collection software v3. Jan 23, 2012 the 3500 series offers themost powerful suite of tools for genetic analysis available. Integrated software for instrument control, data collection, quality control, and autoanalysis of sample files for. Therefore, the applied biosystems 3500 dx genetic analyzer and the applied biosystems 3500xl dx genetic analyzer and the legally marketed device, the applied biosystems 3500 dx3500xl dx genetic analyzer cs2 and 3500 dx series software, are substantially equivalent based on the study data provided in this 510k. Applied biosystems 3500 and 3500xl genetic analyzers. Page 1 applied biosystems 3500 3500xl genetic analyzer user guide with 3500 series data collection software 3. Applied biosystems pop7 polymer for 35003500xl genetic analyzers sample pouch contains pop7 polymer for the 35003500xl genetic analyzer. Abiprism 3100 genetic analyzer definition the abiprism 3100 genetic analyzer is an automated capillary electrophoresis system that can separate, detect, and analyze up to 16 capillaries of fluorescently labeled dna fragments in one run. The 3500 series offers themost powerful suite of tools for genetic analysis available. The applied biosystems 3500 hid genetic analyzer data collection software integrates seamlessly with several downstream software. Powerplexr fusion system for use on the applied biosystems. Applied biosystems 3500 genetic analyzer 8capillary array. The complete system combines the instrument with applied biosystems reagents, consumables, and software, as well as industryleading support providing an. Jan 23, 2017 applied biosystems 3500 series genetic analyzers for dna sequencing and fragment sizing duration.
Applied biosystems 35003500xl genetic analyzers support. Troubleshooting common amplification and ce errorsissues. We have enhanced our goldstandard 3500 series instruments and genemapper idx software solutions in response to customers need to decrease data analysis time, increase confidence in their results, allow for a more efficient processing workflow, and comply with everchanging it requirements. Hid validation includes methods, analysis, results, and conclusions. Applied biosystems 35003500xl genetic analyzer user bulletin. Complete system with pc,computer, user guide and software. Applied biosystems 3500 or 3500xl genetic analyzer section 5.
System software primary analysis with 3500 series data collection software the applied biosystems 3500 and 3500xl genetic analyzers include data collection software with a simple user interface and. System components the 3100 genetic analyzer system includes the following components. Applied biosystems 35003500xl genetic analyzer user guide pn. Use of the msi analysis system with the applied biosystems. Applied biosystems 3500 series genetic analyzers for dna. The basecaller version listed in the basecalling protocol is limited to a 3digit number. The applied biosystems 3500 series genetic analyzers are specifically designed to support the demanding performance needs of validated and regulated environments while retaining the unsurpassed application versatility that life science researchers expect. Regulatory affairs specialist 5781 van allen way tradedevice. Additional updates regarding future plans and changes to the software will be. Combined with our str kits, the improved 3500 data collection software. Applied biosystems 3500 genetic analyzer from thermo.
Thermofisher 3500xl abi 3500xl genetic analyzer for gene. Applied biosystems 35003500xl genetic analyzer user bulletin 5 perform a spatial calibration 1. Click spatial under the calibrate heading on the left of the navigation pane shown below. Designed with you in mind, the 3500 series genetic analyzer gives you automated operation, easytoinstall consumables, electronic tracking of instrument performance and maintenance tasks, and integrated analysis software so you can focus on the outcome. Designed with you in mind, the 3500 dx genetic analyzer cs2 gives you automated operation, easytoinstall consumables, electronic tracking of instrument performance and maintenance tasks, and. Applied biosystems 35003500xl genetic analyzer user guide. Applied biosystems 35003500xl genetic analyzer user. Applied biosystems products offer an affordable yet modern experience, providing you with what you need to enhance your lab productivity. Innovative software, hardware, and consumable development. Therefore, the applied biosystems 3500 dx genetic analyzer and the applied biosystems 3500xl dx genetic analyzer and the legally marketed device, the applied biosystems 3500 dx3500xl dx genetic analyzer cs2 and 3500 dx series software.
Select no fill, or select fill to fill the array with polymer before starting the calibration. Used abi 3500 genetic analyser dna related for sale dotmed. Guidelines for detecting microsatellite instability using the msi analysis system, applied biosystems 3500 genetic analyzer and pop7 polymer. Applied biosystems 3500 genetic analyzer 8capillary array, 36 cm a radiofrequency identification tag is built into the frame for tracking critical run information, which is then reported on the software.
Use the applied biosystems seqstudio genetic analyzer for a wide range of sequencing and fragment analysis applications to deliver highquality results in minutes instead of hours. For the 3500 genetic analyzer, our elearning training modules cover a wide range of topics including fragment analysis, reporting, security, and user management. This newly designed system supports dna sequencing and fragment sizing applications providing scientists with mediumthroughput technology for use in research. Abi prism 3100 genetic analyzer boyce thompson institute. Seqstudio genetic analyzer with data collection software. The 3500 genetic analyzer is an 8capillary sequencing instrument specifically designed to support the demanding performance needs of validated and process controlled. Consumable containers are disposed of when the maximum number of samples has been processed. We set a new standard in capillary electrophoresis with the 8capillary 3500 and 24capillary 3500xl genetic analyzers. Benefits and features of the 3500 genetic analyzer include. The complete product combines the instrument with applied biosystems reagents, consumables and software. Combined with our str kits, the improved 3500 data collection software v4. The 3500series instruments are the first genetic analyzers designed with a specific feature set and workflow for the human identification application. The 3500 series data collection software integrates seamlessly with several downstream software packages to provide comprehensive analysis of genetic data.
The 3500 genetic analyzer is an 8capillary sequencing instrument specifically. The applied biosystems 3500 series instruments were the first genetic analyzers designed with a specific feature set and workflow for human identification hid applications. Argon ion gas lasers, which are used in the abi 310, 3100, and 3 series instruments, have a wider range of power output variability. The applied biosystems 3500 genetic analyzer is an eight capillary electrophoresis system designed for high performance fragment analysis and resequencing applications. It belongs to human identification solutions category.
The 3500 8capillary and the 3500xl 24capillary genetic analysis. Feb 17, 2010 the 3500 series genetic analyzers set a new standard in capillary electrophoresis, with increased throughput, faster run times, and an innovative consumables system approach. The complete product combines the instrument with applied biosystems reagents, consumables and software, as well as industry leading support, to provide an integrated hid system solution that. The maintenance calendar in the applied biosystems 3500 series data collection software. Understanding the consumables on the 3500 genetic analyzers. A genemapper idx software abi prism 3100 or 3100avant genetic analyzer section 5. Based on one of the most widely used, widely trusted sequencing methodologies availablesanger sequencingthe 3500 series genetic analyzer is designed. Designed with you in mind, the 3500 series genetic analyzer gives you automated operation, easytoinstall consumables, electronic tracking of instrument performance and maintenance tasks, and integrated analysis software. Thermo fisher scientific life 3500 genetic analyzer. So today, let me show you the few steps it takes to set up the applied biosystems 3500 genetic analyzer. Genemapper secondary analysis software is included in this package.
Thermofisher 3500xl abi 3500xl genetic analyzer for gene testing, find complete details about thermofisher 3500xl abi 3500xl genetic analyzer for gene testing,abi 3500xl,thermofisher 3500xl,bi 3500xl genetic analyzer. System components the applied biosystems 3500 and 3500xl. The applied biosystems 3500 hid genetic analyzer data collection software integrates seamlessly with several downstream software packages. A genetic analyzer service engineer can vary the power output on the abi 3500 solidstate laser within a specified range and obtain a more consistent fluorescence excitation level. The version listed in sequencing results is a 4digit number. The maintenance calendar in the applied biosystems 3500 series data collection software can provide a monthly or daily view of tasks scheduled for the instrument. Pop4 polymer for 3500 3500xl genetic analyzers stability, and 3500 series buffer stability. This is a capillary array, and it comes with a builtin frame for easy installation. Rfid system of 3500 series which performs automatic detection of reagents and instrument accessories used, forces the instrument operators to replace these reagents and accessories unnecessarily frequently, nonefficiently and makes the whole operation of 3500 series very expensive. There are many differences between the two genetic analyzers, the 3500xl genetic analyzer and 3xl genetic analyzer being compared in this validation study. The 3500 dx genetic analyzer cs2 is an ivdlabeled instrument, making it the first sanger sequencing platform cleared for sequencing analysis of dna.
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